金针菇多糖螯合锌的研究

目录
摘要1
关键词1
Abstract...........1
Key words1
引言1
1材料与主要仪器...............2
1.1试验材料 ..............2
1.1.1金针菇原料的处理.........2
1.1.2主要试剂........................2
1.2主要仪器................................2
2试验方法.......... 3
2.1金针菇多糖的初步分离提取... 3
2.1.1金针菇粗多糖的提取............ 3
2.1.2金针菇粗多糖去蛋白技术................ 3
2.1.3醇沉........................................ 3
2.2金针菇多糖的进一步分离纯化.... 3
2.2.1金针菇多糖DEAE Cellulose52 离子柱层析分离纯化.. 3
2.2.1.1 DEAE Cellulose52 预处理.....................................................................................3
2.2.1.2 装柱...........................................................................................................................3
2.2.1.3 多糖的DEAE Cellulose52 分离纯化与收集........................................................3
2.2.2 金针菇多糖Sephadex G100 凝胶色谱分离纯化.....................................................
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2.2.2.1 Sephadex G100 的预处理......................................................................................4
2.2.2.2 装柱和平衡...............................................................................................................4
2.2.2.3 金针菇多糖分级纯化...............................................................................................4
2.3 多糖的螯合........................................................................................................................4
2.4 多糖的体外抗氧化能力测定............................................................................................5
2.4.1 羟自由基（OH）清除能力的测定 ..................................................................................5
2.4.2 DPPH（1,1二苯基2三硝基苯胫）自由基清除能力测定.........................................5
2.4.3 超氧阴离子自由基(02)清除能力的测定....................................................................5
2.4.4 过氧化氢清除能力的测定.............................................................................................5
3 结果与分析 .........................................................................................................................6
3.1 金针菇多糖的外观...........................................................................................................6
3.1.1 金针菇粗多糖的外观...................................................................................................6
3.1.2 金针菇纯化多糖的外观...............................................................................................6
3.1.3 金针菇螯合锌多糖的外观...........................................................................................6

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