施钾对梨果实中钾转运蛋白和通道蛋白基因表达差异分析
钾是植物生长的必需元素之一，同时被誉为“品质元素”，能够调控植物体内很多重要的生理功能，具有重要的生理作用，如叶片的运动、气孔的张开和闭合及细胞的生长等。植物吸收钾营养的方式有两种钾通道和钾转运蛋白。为研究施钾对植物的影响，本试验采用盆栽的方式，通过设置不同的钾水平（盆栽0（K0）、0.4（K0.4）和 0.8 (K0.8) g/kg土）研究施钾对植物钾含量的影响、对植物生理结构的影响、对光合特性的影响以及对钾转运蛋白基因表达的影响。研究表明伴随施钾量的提高植物体内的钾含量随之提高，施钾增强了植物对K+的吸收能力，同时促进了叶柄和果柄的发育，此外在钾转运蛋白基因的表达方面，在中钾条件下，叶片和果实中AKT1基因表达量分别提高了3.5倍和2.1倍；在高钾条件下，叶片和果实中AKT1基因表达量分别提高了6.2倍和4.8倍；KT12基因在叶片和果实中的表达量在中钾和高钾处理平均分别提高了17.3倍和13.5倍；SKOR基因在叶片和果实中的表达量要比AKT1、KT8和KT12基因的表达量高。因此，我们可以初步判断施钾有利于提高果实的品质，但仍需进一步研究。
目录
摘要 １
关键词 １
Abstract １
引言
引言
1 材料与方法 ２
1.1 试验地点和材料 ２
1.2 试验设计 ２
1.3 栽培管理 ２
1.4 样品采集 ２
1.5 测定项目及方法 ３
1.5.1 叶片、叶柄、果柄和果实中钾离子含量的测定 ３
1.5.2 叶柄和果柄显微结构观察 ３
1.5.3 叶片和果实样品RNA的提取 ３
1.5.4 RealTime PCR检测表达情况 ４
1.5.5 叶片光合特性的测定 ４
1.5.6 数据统计 ４
2 结果与分析 ４
2.1 施钾对不同时期叶片和果实钾含量的影响 ４
2.2 施钾对叶柄和果柄显微结构的影响 ５
2.3 施钾对叶片光合特性的影响 ７
2.4 施钾对叶片和果实中钾离子转运蛋白和通道蛋白基因表达的影响 ８
3 讨论 ９
*好棒文|www.hbsrm.com +Q: *351916072*
/> 3.1 施钾改善植物结构提高果实品质 ９
3.2 施钾调控钾离子转运蛋白基因的表达提高果实品质 ９
致谢 １０
参考文献 １１
施钾对梨果实中钾转运蛋白和通道蛋白基因表达差异分析
Different Expression of Potassium Transporter and Channel in Pear Fruit by Potassium Application
Student majoring in agriculture resource and environment Ruisuhua Liang
Tutor Caixia Dong
Abstract：Potassium (K) is one of the essential elements of plant growth, and is known as a "quality element". It can regulate many important physiological functions in plants. It has important physiological functions, such as the movement of leaves, opening and closing of stomata, and cell growth. There are two ways for plants to absorb K: K channels and K transporters. In order to study the effect of K application on plants, the effects of K fertilizer on plant K content, physiological structure of plants, photosynthetic characteristics and K transporter gene expression were studied by pot cultures experiments setting different potassic levels (pot cultures: 0 (K0), 0.4 (K0.4) and 0.8 (K0.8) g/kg soil). The study showed that the K content of plants increased with the increase of K application. K application enhanced the uptake of K+, and promoted the development of the petiole and fruit stalk. In addition, the expression of AKT1 gene in leaves and fruits increased by 3.5 and 2.1 times. The expression of AKT1 gene in leaves and fruits increased by 6.2 and 4.8 times in high potassium. The expression of KT12 gene in leaves and fruits increased by 17.3 and 13.5 times respectively in the medium potassium and high potassium treatment. The expression of SKOR gene in leaves and fruits was higher than that of AKT1, KT8 and KT12 genes. Therefore, we can preliminarily determine K application is beneficial to improving fruit quality, but we still need further study.
Key words: Potassium ; ‘Huangguan’ Pear; Potassium Transporter; Potassium Channel; Fruit
引言：果实作为强大的库器官，在成熟期时需要大量的营养物质，而叶片作为源则是将大量营养物质和糖物质输送到果实中以维持果实代谢[12]。营养物质主要是通过木质部进行运输，木质部组成分子的结构在系统发育和分类学上颇为重要，因此它在植物组织中占有重要地位。而钾是植物生长的必需元素之一，同时被誉为“品质元素”，也是植物生长发育所需要的三大元素之一，在植物体内的含量仅次于氮，对调节植物生理过程、改善植物生理结构、提高果实品质等有着重要的作用[3]，所以合理运用钾肥对提高果树产量具有重要意义[45]。钾肥在土壤中的形态有四种：矿物质态（占土壤总钾量的90%98%）、非交换性钾、交换性钾以及水溶性钾，其中植物能够直接利用的钾形态为可溶性钾以及交换性钾[6]。
目录
摘要 １
关键词 １
Abstract １
引言
引言
1 材料与方法 ２
1.1 试验地点和材料 ２
1.2 试验设计 ２
1.3 栽培管理 ２
1.4 样品采集 ２
1.5 测定项目及方法 ３
1.5.1 叶片、叶柄、果柄和果实中钾离子含量的测定 ３
1.5.2 叶柄和果柄显微结构观察 ３
1.5.3 叶片和果实样品RNA的提取 ３
1.5.4 RealTime PCR检测表达情况 ４
1.5.5 叶片光合特性的测定 ４
1.5.6 数据统计 ４
2 结果与分析 ４
2.1 施钾对不同时期叶片和果实钾含量的影响 ４
2.2 施钾对叶柄和果柄显微结构的影响 ５
2.3 施钾对叶片光合特性的影响 ７
2.4 施钾对叶片和果实中钾离子转运蛋白和通道蛋白基因表达的影响 ８
3 讨论 ９
*好棒文|www.hbsrm.com +Q: *351916072*
/> 3.1 施钾改善植物结构提高果实品质 ９
3.2 施钾调控钾离子转运蛋白基因的表达提高果实品质 ９
致谢 １０
参考文献 １１
施钾对梨果实中钾转运蛋白和通道蛋白基因表达差异分析
Different Expression of Potassium Transporter and Channel in Pear Fruit by Potassium Application
Student majoring in agriculture resource and environment Ruisuhua Liang
Tutor Caixia Dong
Abstract：Potassium (K) is one of the essential elements of plant growth, and is known as a "quality element". It can regulate many important physiological functions in plants. It has important physiological functions, such as the movement of leaves, opening and closing of stomata, and cell growth. There are two ways for plants to absorb K: K channels and K transporters. In order to study the effect of K application on plants, the effects of K fertilizer on plant K content, physiological structure of plants, photosynthetic characteristics and K transporter gene expression were studied by pot cultures experiments setting different potassic levels (pot cultures: 0 (K0), 0.4 (K0.4) and 0.8 (K0.8) g/kg soil). The study showed that the K content of plants increased with the increase of K application. K application enhanced the uptake of K+, and promoted the development of the petiole and fruit stalk. In addition, the expression of AKT1 gene in leaves and fruits increased by 3.5 and 2.1 times. The expression of AKT1 gene in leaves and fruits increased by 6.2 and 4.8 times in high potassium. The expression of KT12 gene in leaves and fruits increased by 17.3 and 13.5 times respectively in the medium potassium and high potassium treatment. The expression of SKOR gene in leaves and fruits was higher than that of AKT1, KT8 and KT12 genes. Therefore, we can preliminarily determine K application is beneficial to improving fruit quality, but we still need further study.
Key words: Potassium ; ‘Huangguan’ Pear; Potassium Transporter; Potassium Channel; Fruit
引言：果实作为强大的库器官，在成熟期时需要大量的营养物质，而叶片作为源则是将大量营养物质和糖物质输送到果实中以维持果实代谢[12]。营养物质主要是通过木质部进行运输，木质部组成分子的结构在系统发育和分类学上颇为重要，因此它在植物组织中占有重要地位。而钾是植物生长的必需元素之一，同时被誉为“品质元素”，也是植物生长发育所需要的三大元素之一，在植物体内的含量仅次于氮，对调节植物生理过程、改善植物生理结构、提高果实品质等有着重要的作用[3]，所以合理运用钾肥对提高果树产量具有重要意义[45]。钾肥在土壤中的形态有四种：矿物质态（占土壤总钾量的90%98%）、非交换性钾、交换性钾以及水溶性钾，其中植物能够直接利用的钾形态为可溶性钾以及交换性钾[6]。
版权保护: 本文由 hbsrm.com编辑，转载请保留链接: www.hbsrm.com/hxycl/zyyhj/255.html
