月季种质资源保存技术研究
目录
1 引言 1
2 研究目的 1
3 研究意义 1
4 研究内容 2
4.1实验材料 2
4.2 实验方法 2
4.2.1 配置MS培养基（mg/l） 2
4.2.2 配置生长调节剂母液 3
4.2.3 配置培养基 3
4.2.4 培养基的消毒灭菌 4
4.2.5 外植体的选择与消毒 4
4.2.6 外植体接种 4
4.2.7 月季继代分化 5
4.2.8 月季生根培养 5
4.2.9 配置限制生长培养基 5
5 研究结果 5
5.1 不同浓度的生长激素对于月季不定芽增殖率的影响 5
5.2灭菌药品消毒时间不同对月季成活率的影响 8
5.3 不同部位的茎段对月季芽分化的影响 8
5.4 不同浓度的MS培养基对月季生根培养的影响 10
5.5 不同浓度的限制培养基对月季生长的抑制作用 10
6 总结 12
7讨论 12
致谢 14
参考文献 15
毕业设计说明书（论文）中文摘要
植物的种质资源保存技术在当今世界具有重要作用。本文研究了月季种质资源保存技术中离体培养的操作方法。通过培养基浓度的对比，外植体的选择，灭菌材料以及时间的对比，限制生长的植物激素激素浓度的对比，探索月季种质资源保存技术中的最优的种质资源保存方法，并且提出了在月季组织培养中出现的问题及建议，为月季的种质资源保存技术提供实践基础。 结果表明：在月季的发芽阶段，当NAA浓度为0.2mg/l时，选用1.5mg/l的MS+6- *好棒文|www.hbsrm.com +Q: 3 5 1 9 1 6 0 7 2
BA月季不定芽增殖率最高。MS+6-BA浓度在1.5mg/l~2mg/l时，月季增殖率最高。消毒过程中，75%酒精消毒2min时，月季成活率最高，为78%，2%次氯酸钠消毒6min，月季成活率最高为83%。0.1%升汞消毒4min，月季成活率最高为84%。在月季外植体的选择上，选用中段外植体诱导分化率较基部与梢部高，并且，中段的外植体生长速度最快。在月季生根阶段，选择1/2MS+0.5mg/l NAA+AC效果最佳。在抑制生长阶段，在不同浓度的ABA抑制效果实验对比中，选用1mg/l的ABA，对月季生长抑制效果最佳，而在不同浓度的CCC抑制效果实验对比中，选用40mg/l的CCC对月季生长抑制效果最佳。 关键词 月季，种质资源保存，组织培养，限制生长
毕业设计说明书（论文）外文摘要
Title Study On the Technology of Preservation of Rose Germplasm Abstract Plant germplasm conservation technology plays an important role in todays world. In this paper, the operation method of the in vitro culture of rose germplasm resource preservation was studied. By cultivating the basal concentrations of contrast, selection of explants, sterilization materials and compare the time, limit plant growth hormone concentration of contrast, explore rose germplasm resources preservation technology of optimal germplasm resources preservation methods, and puts forward the problems and suggestions in the tissue culture of Rosa chinensis, rose germplasm resources preservation technology to provide practical basis. The result shows that the rose of the germination stage, when concentration of NAA 0.2mg, choose 1.5mg/l MS + 6-BA rose not shoot proliferation rate was the highest. MS+6-BA concentration in 1.5mg/l~2mg/l, the highest proliferation rate. The disinfection process, 75% alcohol disinfection for 2 min, *好棒文|www.hbsrm.com +Q: 3 5 1 9 1 6 0 7 2
rose survival rate highest was 78%, 2% sodium hypochlorite disinfection 6min, rose survival rate was highest for 83%. 0.1% mercuric chloride for 4min, the highest survival rate rose 84%. On the selection of Chinese rose, the differentiation rate of the middle segment was higher than that of the basal and shoot, and the growth rate of the explants was the fastest. In the rooting stage, the selection of NAA+AC 1/2MS+0.5mg/l effect is the best. In suppressing the growth stage, in different concentrations of ABA inhibition effect experimental comparison and selection of 1mg / L ABA, of rose growth inhibition effect is the best, and in different concentrations of CCC inhibition effect experimental comparison, the choice of 40mg / L CCC of rose growth inhibition effect is the best. Keywords Rose, germplasm preservation, tissue culture, growth constraints
月季组织培养，取芽时要在无病虫害的优良品种单株上进行，选取生长健壮的当年生枝条作为外植体，将取回的材料用手术刀片切去叶片及叶柄，切成1-2cm的带芽茎段，将清理好的材料在自来水下冲洗干净，后用洗衣粉浸泡5min，再用流水冲洗10min，洗去泡沫。然后在无菌条件下用75%酒精表面消毒，倒出酒精，无菌水冲洗，然后用2%的次氯酸钠溶液中浸泡，倒出次氯酸钠，无菌水冲洗，最后加入0.1%的升汞溶液灭菌，取出茎段，在无菌水中清洗2-4次。消毒时间进行对比实验，选择合适的消毒方案。在整个灭菌和清洗过程总，应不停地摇动瓶子，使其充分与消毒液接触，否则会影响消毒和灭菌效果，灭菌后的外植体取出后用无菌滤纸吸干水分待用。每种消毒剂接种5瓶，每瓶接种芽5个，以25个芽为样本，选择污染率较低的一种方式。
1 引言 1
2 研究目的 1
3 研究意义 1
4 研究内容 2
4.1实验材料 2
4.2 实验方法 2
4.2.1 配置MS培养基（mg/l） 2
4.2.2 配置生长调节剂母液 3
4.2.3 配置培养基 3
4.2.4 培养基的消毒灭菌 4
4.2.5 外植体的选择与消毒 4
4.2.6 外植体接种 4
4.2.7 月季继代分化 5
4.2.8 月季生根培养 5
4.2.9 配置限制生长培养基 5
5 研究结果 5
5.1 不同浓度的生长激素对于月季不定芽增殖率的影响 5
5.2灭菌药品消毒时间不同对月季成活率的影响 8
5.3 不同部位的茎段对月季芽分化的影响 8
5.4 不同浓度的MS培养基对月季生根培养的影响 10
5.5 不同浓度的限制培养基对月季生长的抑制作用 10
6 总结 12
7讨论 12
致谢 14
参考文献 15
毕业设计说明书（论文）中文摘要
植物的种质资源保存技术在当今世界具有重要作用。本文研究了月季种质资源保存技术中离体培养的操作方法。通过培养基浓度的对比，外植体的选择，灭菌材料以及时间的对比，限制生长的植物激素激素浓度的对比，探索月季种质资源保存技术中的最优的种质资源保存方法，并且提出了在月季组织培养中出现的问题及建议，为月季的种质资源保存技术提供实践基础。 结果表明：在月季的发芽阶段，当NAA浓度为0.2mg/l时，选用1.5mg/l的MS+6- *好棒文|www.hbsrm.com +Q: 3 5 1 9 1 6 0 7 2
BA月季不定芽增殖率最高。MS+6-BA浓度在1.5mg/l~2mg/l时，月季增殖率最高。消毒过程中，75%酒精消毒2min时，月季成活率最高，为78%，2%次氯酸钠消毒6min，月季成活率最高为83%。0.1%升汞消毒4min，月季成活率最高为84%。在月季外植体的选择上，选用中段外植体诱导分化率较基部与梢部高，并且，中段的外植体生长速度最快。在月季生根阶段，选择1/2MS+0.5mg/l NAA+AC效果最佳。在抑制生长阶段，在不同浓度的ABA抑制效果实验对比中，选用1mg/l的ABA，对月季生长抑制效果最佳，而在不同浓度的CCC抑制效果实验对比中，选用40mg/l的CCC对月季生长抑制效果最佳。 关键词 月季，种质资源保存，组织培养，限制生长
毕业设计说明书（论文）外文摘要
Title Study On the Technology of Preservation of Rose Germplasm Abstract Plant germplasm conservation technology plays an important role in todays world. In this paper, the operation method of the in vitro culture of rose germplasm resource preservation was studied. By cultivating the basal concentrations of contrast, selection of explants, sterilization materials and compare the time, limit plant growth hormone concentration of contrast, explore rose germplasm resources preservation technology of optimal germplasm resources preservation methods, and puts forward the problems and suggestions in the tissue culture of Rosa chinensis, rose germplasm resources preservation technology to provide practical basis. The result shows that the rose of the germination stage, when concentration of NAA 0.2mg, choose 1.5mg/l MS + 6-BA rose not shoot proliferation rate was the highest. MS+6-BA concentration in 1.5mg/l~2mg/l, the highest proliferation rate. The disinfection process, 75% alcohol disinfection for 2 min, *好棒文|www.hbsrm.com +Q: 3 5 1 9 1 6 0 7 2
rose survival rate highest was 78%, 2% sodium hypochlorite disinfection 6min, rose survival rate was highest for 83%. 0.1% mercuric chloride for 4min, the highest survival rate rose 84%. On the selection of Chinese rose, the differentiation rate of the middle segment was higher than that of the basal and shoot, and the growth rate of the explants was the fastest. In the rooting stage, the selection of NAA+AC 1/2MS+0.5mg/l effect is the best. In suppressing the growth stage, in different concentrations of ABA inhibition effect experimental comparison and selection of 1mg / L ABA, of rose growth inhibition effect is the best, and in different concentrations of CCC inhibition effect experimental comparison, the choice of 40mg / L CCC of rose growth inhibition effect is the best. Keywords Rose, germplasm preservation, tissue culture, growth constraints
月季组织培养，取芽时要在无病虫害的优良品种单株上进行，选取生长健壮的当年生枝条作为外植体，将取回的材料用手术刀片切去叶片及叶柄，切成1-2cm的带芽茎段，将清理好的材料在自来水下冲洗干净，后用洗衣粉浸泡5min，再用流水冲洗10min，洗去泡沫。然后在无菌条件下用75%酒精表面消毒，倒出酒精，无菌水冲洗，然后用2%的次氯酸钠溶液中浸泡，倒出次氯酸钠，无菌水冲洗，最后加入0.1%的升汞溶液灭菌，取出茎段，在无菌水中清洗2-4次。消毒时间进行对比实验，选择合适的消毒方案。在整个灭菌和清洗过程总，应不停地摇动瓶子，使其充分与消毒液接触，否则会影响消毒和灭菌效果，灭菌后的外植体取出后用无菌滤纸吸干水分待用。每种消毒剂接种5瓶，每瓶接种芽5个，以25个芽为样本，选择污染率较低的一种方式。
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