卤代对羟基苯甲酸的微生物降解机制

目录
摘要.................................................................................3
关键词...............................................................................3
Abstract.............................................................................3
Key words............................................................................3
绪论.................................................................................4
1材料与方法..........................................................................4
1.1菌株..............................................................................4
1.2培养基与试剂......................................................................4
1.3DBHB与CHB检测方法.............................................................5
1.3.1紫外分光光度法..................................................................5
1.3.2高效液相色谱定性定量检测法......................................................5
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1.4菌株H8降解DBHB与CHB的途径研究.................................................5
1.4.1中间代谢物收集...................................................................5
1.4.2样品处理.........................................................................5
1.4.3质谱检测.........................................................................5
1.5菌株H8降解DBHB与CHB的诱导实验.................................................6
1.6比较蛋白组学分析..................................................................6
1.6.1细菌总蛋白提取...................................................................6
1.6.2总蛋白浓度测定...................................................................6
1.6.3总蛋白酶解消化...................................................................7
1.6.4iTraq标记酶解肽段................................................................7
1.6.5强阳离子交换色谱分级.............................................................7
1.6.6液相串联质谱鉴定分析.............................................................7
2 结果与分析.........................................................................8
2.1菌株H8降解DBHB代谢途径研究.....................................................8
2.1.1DBHB中间代谢产物收集...........................................................8
2.1.2DBHB中间代谢产物的质谱鉴定分析.................................................8
2.2菌株H8降解CHB代谢途径研究.......................................................9
2.2.1CHB中间代谢产物收集.............................................................9
2.2.2LCMS/MS对CHB中间代谢产物的鉴定分析.........................................10
2.3 菌株H8降解DBHB与CHB的诱导实验...............................................10
2.4比较蛋白组学分析.................................................................11
2.4.1DBHB降解基因推测..............................................................12
2.4.2CHB降解基因推测................................................................14

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